nsclc tumor  (OriGene)

Journal: EMBO reports

Article Title: miR-515-5p controls cancer cell migration through MARK4 regulation.

doi: 10.15252/embr.201540970

Figure Lengend Snippet: Figure 4. MARK4 overexpression rescues the inhibitory effects of miR-515-5p on cell migration.

Article Snippet: Lung cancer versus normal lung cDNA arrays The levels for MARK4 mRNA were determined in the HLRT104 TissueScan cDNA array (Origene) containing 24 lung cancer and matching normal lung samples and were analysed by using the Fast SYBR Green PCR Master Mix and 7900HT Real-time PCR System (Applied Biosystems).

Techniques: Over Expression, Migration

Journal: EMBO reports

Article Title: miR-515-5p controls cancer cell migration through MARK4 regulation.

doi: 10.15252/embr.201540970

Figure Lengend Snippet: Figure 7. miR-515-5p overexpression or siRNA-mediated MARK4 silencing prevents A549 cell metastasis to the lung. A549-Luc cells transfected either with miR-515-5p precursor (miR), siRNAs to MARK4 (siMARK4) or the corresponding control sequences (C and NT, respectively) were injected in SCID mice through the tail vein. Four weeks later, the mice were sacrificed and their lungs extracted and fixed prior to staining for luciferase expression.

Article Snippet: Lung cancer versus normal lung cDNA arrays The levels for MARK4 mRNA were determined in the HLRT104 TissueScan cDNA array (Origene) containing 24 lung cancer and matching normal lung samples and were analysed by using the Fast SYBR Green PCR Master Mix and 7900HT Real-time PCR System (Applied Biosystems).

Techniques: Over Expression, Transfection, Control, Injection, Staining, Luciferase, Expressing

Journal: Oncogene

Article Title: The Hedgehog processing pathway is required for NSCLC growth and survival

doi: 10.1038/onc.2012.243

Figure Lengend Snippet: a, SKN and b, DISP-1 are differentially expressed in NSCLCs as compared to the corresponding matched normal lung tissue. Expression levels from 58 matched NSCLC/normal lung tissue pairs were assessed by oligonucleotide micro-array analyses. Gene expression in NSCLC was normalized to matched normal tissue and plotted as the Log10 change (tumor/normal). c, SKN is overexpressed in commercially available clinical lung cancer specimens, confirming the pattern of expression detected by micro-array analysis. These were purchased from Origene (Tissuescan Lung Cancer cDNA Array IV) and consist of 24 matched lung cancer/normal lung cDNA pairs pre-normalized to β-ACTIN. SKN expression was determined by qRT-PCR, and lung tumor expression was normalized to matched normal control. d, DISP-1 is differentially expressed in clinical lung cancer specimens. The same Tissuescan cDNA array was used to assess DISP-1 levels by qRT-PCR. Arrows represent pairs where DISP-1 expression was below the threshold of detection in either normal lung tissue (up arrow) or lung tumor (down arrow). e, Higher DISP-1 expression is associated with reduced recurrence free survival. A publically available dataset (GSE8894) consisting of micro-array profiles of 138 NSCLC cases and an associated clinical survival database (recurrence-free survival) was mined to find correlations between DISP-1 expression and recurrence free survival. Lung cancers were separated into tertiles based on DISP-1 expression, and the top third compared to the bottom third. f, Higher DISP-1 expression is associated with reduced survival in a second publically available dataset (GSE10245) consisting of micro-array profiles of 58 NSCLC cases and an associated clinical outcome database (overall and progression-free survival). This dataset was analyzed in the same manner as for GSE10245. P-values reported are from a one-tailed Log-rank (Mantel-Cox) test.

Article Snippet: These were purchased from Origene (Tissuescan Lung Cancer cDNA Array IV) and consist of 24 matched lung cancer/normal lung cDNA pairs pre-normalized to β- ACTIN .

Techniques: Expressing, Microarray, Quantitative RT-PCR, One-tailed Test